Development of an Unmarked Gene Deletion System for the Filamentous Fungi Aspergillus niger and Talaromyces versatilis
نویسندگان
چکیده
منابع مشابه
Development of an unmarked gene deletion system for the filamentous fungi Aspergillus niger and Talaromyces versatilis.
In this article, we present a method to delete genes in filamentous fungi that allows recycling of the selection marker and is efficient in a nonhomologous end-joining (NHEJ)-proficient strain. We exemplify the approach by deletion of the gene encoding the transcriptional regulator XlnR in the fungus Aspergillus niger. To show the efficiency and advantages of the method, we deleted 8 other gene...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2014
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.00625-14